nadh absorbance at 340 nm

Cashback Di Natale, Qf Ml@DEHb!(`HPb0dFJ|yygs{. Compare calibration curves using Abs and Fluorescence. Lets briefly consider benzene and pyridine. e.g. A NADH preparation was so obtained for which the 260 nm/340 nm absorbance ratio was 2.265. !bRe*)],>TL1h)?Un\Lp;ZMgIC.p 0rjGq(gJ)gDAiTqV@6iXL4DjZV^4$,!@0K'9V"0mK,=n*qsio&0'Gql9h0nIIg"[loXKNdX? 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MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Worksheets:_General_Chemistry" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Worksheets:_Inorganic_Chemistry" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Worksheets:_Organic_Chemistry" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Worksheets:_Physical_Chemistry" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, Absorbance and Fluorescence Analysis of NAD and NADH, [ "article:topic", "fluorescence", "NAD", "license:ccbyncsa", "licenseversion:40", "authorname:asdl" ], https://chem.libretexts.org/@app/auth/3/login?returnto=https%3A%2F%2Fchem.libretexts.org%2FAncillary_Materials%2FWorksheets%2FWorksheets%253A_Analytical_Chemistry_II%2FAbsorbance_and_Fluorescence_Analysis_of_NAD_and_NADH, \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}}}\) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash{#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\), https://www.biotek.com/resources/application-notes/determining-nadh-concentrations-with-synergy-2-multi-mode-microplate-reader-using-fluorescence-or-absorbance/, status page at https://status.libretexts.org. )@taXPGkf7tVJRq2P9fbG.IlotnV`B#g0@2r8`u[Dj5K%Y(E/^K \<9OLcTJ1*NC=&h>6@T[07! When on the institution site, please use the credentials provided by your institution. The absorbance spectrum for NAD and NADH is shown below. 0000003460 00000 n What could you do in the lab to solve this problem?

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