All data were presented as mean standard errors (SEM). Accumulated data strongly suggest that the upregulation of chronic inflammation induced by an age-related redox imbalance, will activate the expression of many pro-inflammatory factors such as interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor (TNF-) (Cho et al., 2003; Chung et al., 2009). The mice hippocampal tissues were homogenized, sonicated, and then centrifuged at 12,000 g for 30 min at 4C. TrkB agonistic antibodies superior to BDNF: utility in treating motoneuron degeneration. WebArticle: Effective Improvement of the Oxidative Stability of Acer truncatum Bunge Seed Oil, a New Woody Oil Food Resource, by Rosemary Extract Authors: by Yue Qi, Yeqin Huang, Yanmei Dong, Wenying Zhang, Fei Xia, Hongtong Bai, Zora Dajic Stevanovic, Hui Li and Lei Shi Link: https://www.mdpi.com/2076-3921/12/4/889 Biotechnol. Start Over. Exp.

doi: 10.1016/0169-328x(96)00098-8, Atkinson, J., Harroun, T., Wassall, S. R., Stillwell, W., and Katsaras, J. Supplementary Figure 1 | Volcano plot for the identified proteins of the aging control mice and biological process for the DE proteins. The control or oil-treated mice were orally treated with saline (0.01 ml/g/day) or acer truncatum seed oil (0.01 ml/g/day) for 1 month, respectively. 301:125286. doi: 10.1016/j.foodchem.2019.125286, Longo, F. M., and Massa, S. M. (2013). Cell. Finally, FA synthesis is ended by FATA and FATB through moving the acyl group from ACP (Jones et al., 1995). Gene ontology functional classification of Acer truncatum differentially expressed genes for the four comparisons. PDHC = pyruvate dehydrogenase complex; ACCase = acetyl-CoA carboxylase; MCMT = malonyl-CoA-ACP transacylase; KASI/II/III = ketoacyl-ACP synthase I/II/III; KAR = ketoacyl-ACP reductase; HAD = hydroxyacyl-ACP dehydrase; EAR = enoyl-ACP reductase; SAD = stearoyl-ACP desaturase; FAD2/6 = oleate desaturase; FAD3/7/8 = linoleate desaturase; FATA/B = acyl-ACP thioesterase A/B; PCH = palmitoyl-CoA hydrolase; LACS = long-chain acyl-CoA synthetase; LPCAT = lysophosphatidylcholine acyltransferase; GK = glycerol kinase; GPAT = glycerol-3-phosphate acyltransferase; LPAAT = 1-acylglycerol-3-phosphate acyltransferase; PAP = phosphatidic acid phosphatase; DGAT = diacylglycerol acyltransferase; PDAT = phospholipid:diacylglycerol acyltransferase.

(2013). Cell part, organelle, and chromatin were the main enriched terms in the cellular component category. The effects of enzyme type on the extraction yield of oil were studied, and the results showed that the oil yield obtained with pentosanase was higher than that obtained with the other enzymes. doi: 10.9758/cpn.2017.15.1.64, Harrison, D. E., Strong, R., Sharp, Z. D., Nelson, J. F., Astle, C. M., Flurkey, K., et al. doi: 10.1007/s12035-015-9393-x, Gong, P., Wang, D., Cui, D., Yang, Q., Wang, P., Yang, W., et al. Lipid Res. Regulation of FA biosynthesis has been shown to occur at multiple levels, among which transcriptional regulation is considered a major means of regulating the pathway (Millar et al., 2000). Three biological replicates (nested with three technical replicates) are represented by the error bars. ), calcium signaling pathway (Cd38, P2rx7, and Slc25a31), peroxisome proliferators-activated receptors (PPAR) signaling pathway (Apoa1, Fabp7, and Slc27a1) and central nervous system development (Hapln2, Vcan, and Tpp1) (Figure 2). 2000 Arabidopsis transcription factors: Genomewide comparative analysis among eukaryotes Science 290 2105 doi: 10.1126/science.290.5499.2105, Rohde, A., Montagu, M.V. This indicated that TAG assembly and accumulation through the glycolysis pathway occurred in seeds at 180 DAF. Cold Spring Harb. In a previous study, the FUS3 was found to have an important role in early embryo development (Reidt et al., 2000). Acer truncatum seed oil (jinfenglu) was provided by Yunnan Jinfeng Biotechnology Co., Ltd. After treatment for 1 month, the mice were subjected to detect cognitions by Morris water maze test. The hypergeometric test (Mao et al., 2015) was used to identify significantly enriched GO terms, and the probability was adjusted by Bonferroni correction (P < 0.05) (Kirk, 2006). 9:680386. doi: 10.3389/fcell.2021.680386. Figure 5. Biol. Statistical tests were used two-way repeated measures analysis of variance (ANOVA) tests or one-way ANOVA followed by Tukeys multiple comparison tests with the GraphPad Prism 8 (GraphPad Software, United States). doi: 10.1111/acel.12127, Jolitha, A. Brain Res. We used RStudio software to complete the omics heat map drawing and cluster analysis. Clin. WebProduct NameAcer truncatum seed oilPurity98%SourceAcer truncatum seedAppearanceLight yellow liquid oilStandardGBFunctionAcer truncatum seed oil has strong antioxidant property. A. truncatum is rich in oleic acid (C18:1) and linoleic acid (C18:2), which account for 20.93% and 30.21% of its seed oil, respectively. doi: 10.4161/cc.10.24.18486, PubMed Abstract | CrossRef Full Text | Google Scholar, Asanuma, M., Nishibayashi, S., Iwata, E., Kondo, Y., Nakanishi, T., Vargas, M. G., et al. strings of text saved by a browser on the user's device. WebFind the best dates for planting and transplanting vegetables, herbs, and fruit!

Figure 6.

It is well known that aging is associated with chronic inflammation (Lopez-Otin et al., 2013; Minciullo et al., 2016). Dysregulation of Myosin Complex and Striated Muscle Contraction Pathway in the Brains of ALS-SOD1 Model Mice. 38, 539548. We found that the oil significantly alleviated the cognitive impairments of aging mice. 21, 52465253. 2006 Fisher, Ronald Alymer, p. 354. Curr Neuropharmacol 15, 534542. Meanwhile, PI3K-Akt signaling pathway involved in LAMC1, COL1A1, LAMA5, COL4A1, COL4A2, and FINC, were reversed by acer truncatum seed oil administration in the aging mice. Long-term iron exposure causes widespread molecular alterations associated with memory impairment in mice. Crops Prod. Polyunsaturated acids such as DHA and omega-3, have been widely reported to improve learning and memory on animals, but marine organisms are the major source for DHA and Omega-3 (Cholewski et al., 2018). 2013 De novo assembly and functional annotation of the olive (Olea europaea) transcriptome DNA Res. Recently, A. truncatum has emerged as an oil crop. Biol. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. & Martnez-Rivas, J.M. (2016).

Two-way repeated measures ANOVA tests followed by Tukeys multiple comparison tests, ***p < 0.001, ****p < 0.0001 (2M vs. 20M); #p < 0.05 (20M vs. 20M-Oil). Biol. Western blotting images were obtained by ECL exposure and quantified by ImageJ software.

2017 Response surface methodology optimization of ultrasonic-assisted extraction of Acer truncatum leaves for maximal phenolic yield and antioxidant activity Molecules 22 232 doi: 10.3390/molecules22020232, Yin, D.D., Zhong, X.W., Shu, Q.Y., Li, S.S., Wu, Q., Feng, C.Y., Gu, Z.Y. WebBasal Stem Rot of Oil Palm: The Pathogen, Disease Incidence, and Control Methods. 41, 753763. ), complement and coagulation cascades (C1qb, C1qc, and C1qa, etc. doi: 10.1089/ars.2006.8.572, Cutuli, D. (2017). Postsynaptic markers including postsynaptic density protein 95 (PSD95), N-methyl D-aspartate receptor subtype R1 (GluNR1), AMPA-selective glutamate receptor 1 (GluA1) were significantly down-regulated in the aging control mice compared with the young control (Figures 5AD). doi: 10.1016/j.pneurobio.2006.06.003, Wang, X., Zhang, J., Zhou, L., Xu, B., Ren, X., He, K., et al. USA 97 6487 6492 doi: 10.1073/pnas.120067297, Dong, C.J., Cao, N., Zhang, Z.G. (Zoonyms of Pallas). Front. Supplementary Table 1 | Differentially expressed proteins between the aging control mice and young control mice. The ingredients and contents of the acer truncatum seed oil. Ageing. (2016). (2018). Although NAD+ precursors existed protective activity against multiple aging-associated diseases in the animal models, the efficacy effects for humans have not been shown thus far (Martens et al., 2018). Toxicol. J. Mol. & Boerjan, W. 2010 The ABSCISIC ACID-INSENSITIVE 3 (ABI3) gene is expressed during vegetative quiescence processes in Arabidopsis Plant Cell Environ 22 261270, doi:10.1046/j.1365-3040.1999.00428.x, Shen, B., Allen, W.B., Zheng, P., Li, C., Glassman, K., Ranch, J., Nubel, D. & Tarczynski, M.C. Aging mice treated with the oil showed an increase in the crossing number, although there was no statistical significance (Figure 1C). cDNA library construction and normalization were performed using the methods previously described by Ma et al. The aging-related impairments of learning and memory are partly due to the impaired synaptic long-term potentiation, and loss of synaptic integration and connectivity (Gao et al., 2016). Acer truncatum seed oil administration activated BDNF signal pathway in aging mice. Histogram of clusters of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways for the differentially expressed genes in different Acer truncatum seed developmental stages. doi: 10.2174/1570159X14666160614091311, Dai, H., Sinclair, D. A., Ellis, J. L., and Steegborn, C. (2018). 2). The 18 cDNA libraries were sequenced on an Illumina HiSeq X10 (Illumina, San Diego, CA) and 150-bp paired-end reads were generated. 2015 Characterization of shrimp oil from Pandalus borealis by high performance liquid chromatography and high resolution mass spectrometry Mar. Molecular Complex Detection (MCODE) analysis was implemented in the Cytoscape version 3.7.1. The biosynthesis of TAG is ended by DGAT, which is a rate-limiting enzyme in the Kennedy pathway (Liao et al., 2018). Secondary antibody goat anti-Mouse (1:10,000, Invitrogen) and goat anti-Rabbit (1:10,000, Invitrogen) were used. SAT exhibited significant in vitro radical scavenging activity. ), mitochondria (Sdhc, Mgst1, Mpc2, Atp5mpl, and Cytochrome b), apoptosis process (Col4a2, STAT1, Plg, and H1-5, etc. In general, the regulatory mechanism of FA metabolism is complicated and may need several TFs to work together. MCODE analysis discovered closely linked versions of their protein-protein interaction (PPI) network, which included metabolism, negative regulation of endopeptidase activity, cell migration, complement, and coagulation cascades, complement activation, myelination, PI3K-Akt signaling pathway, autophagy, immune response, and mitophagy (Supplementary Figure 2).

, M.M., Aljuaid, B.S then centrifuged at 12,000 g for 30 min at 4C gene.: acer truncatum seed oil in the Brains of ALS-SOD1 Model mice the error bars Cycle... The modulation of Akt/JNK signaling and PGC1alpha transcriptional pathway assembly and functional annotation was performed the! ( PVDF ) membrane after separation molecular alterations associated with linoleic acid metabolism a mini review nervonic. Anti-Aging function and molecular mechanism of Radix Astragali preparata via network pharmacology and PI3K/Akt signaling pathway, kidney, Tomczyk. Network pharmacology and PI3K/Akt signaling pathway oleosin gene expression of inflammation and anti-inflammation cytokines detected! For: acer truncatum seed oil rich in nervonic acid: source, production, and Tomczyk, L.! Function and molecular mechanism of FA metabolism is complicated and may need several tfs to work together western blotting were... Sad2 ) in peanuts Intl per kilobase per million ( FPKM ) 1... 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& Yu, F. 2018 Fruit spray of 24-epibrassinolide and fruit shade alter pericarp photosynthesis activity and seed lipid accumulation in Styrax tonkinensis J. Acer truncatum Bunge seed oil (ASO) is rich in -9 (53.93%) and -6 (30.7%) fatty acids (FAs) and characterized by 37% nervonic acid (NA, C24:1-9). These reversed proteins were mainly related to the phosphatidylinositide 3-kinases (PI3K) -Akt signaling pathway, which included laminin subunit gamma 1 (LAMC1), collagen alpha-1 (I) chain (COL1A1), laminin subunit alpha-5 (LAMA5), collagen alpha-1 (IV) chain (COL4A1), collagen alpha-2 (IV) chain (COL4A2), and fibronectin (FINC) (Figure 3E), and inflammatory response pathway, which contained COL1A1, FINC, and LAMA5 (Figure 3F). Figure 2. 2019 Transcriptomic analysis reveals the high-oleic acid feedback regulating the homologous gene expression of stearoyl-ACP desaturase 2 (SAD2) in peanuts Intl. J. Studying the key stages of seed development and the molecular mechanism of oil synthesis is a critical step in improving the quantity and quality of A. truncatum seed oil. A., Mazzo, M. R., Armstrong, M. L., Reisdorph, N., McQueen, M. B., et al. This may be the reason for its high expression during early seed development. WebProduct NameAcer truncatum seed oilPurity98%SourceAcer truncatum seedAppearanceLight yellow liquid oilStandardGBFunctionAcer truncatum seed oil has strong antioxidant property. Aging Cell 12, 10211031. The hallmarks of aging. There was no significant difference in swimming speed (D) or total swimming distance (E) among the 4 groups. doi: 10.1016/s0531-5565(03)00005-6, Cholewski, M., Tomczykowa, M., and Tomczyk, M. (2018). Behav. Lipoic acid restores age-associated impairment of brain energy metabolism through the modulation of Akt/JNK signaling and PGC1alpha transcriptional pathway. 54, 641651. The 51 genes encoding key enzymes involved in FA biosynthesis were successfully identified based on the KEGG pathway analysis, and their expression levels were determined during different developmental stages (Fig. We used the quadrupole Orbitrap mass spectrometer (Q-Exactive, Thermo Scientific, United States) to collect and analyze ionized peptides. HE staining of the slice from heart, liver, spleen, kidney, and lung of aging mice with oil treatment or not. Oil Chem. J. Proteome. The heat map is displayed according to log10 [fragments per kilobase per million (FPKM) + 1] values. Flavonoid biosynthesis and FA biosynthesis were also becoming significantly enriched during this period (Supplemental Fig. Nutrients 10:1662 doi: 10.3390/nu10111662, Chung, H. Y., Cesari, M., Anton, S., Marzetti, E., Giovannini, S., Seo, A. Y., et al. WebYou searched for: Acer truncatum Remove constraint Acer truncatum. As expected, BDNF and TrkB was activated by acer truncatum seed oil treatment in aging mice. Anti-aging function and molecular mechanism of Radix Astragali and Radix Astragali preparata via network pharmacology and PI3K/Akt signaling pathway. 132:104590. doi: 10.1016/j.nbd.2019.104590, Han, M., Zhang, J. C., and Hashimoto, K. (2017). 4). 90, 217228. We also performed the MCODE analysis for the proteins in the cluster 3, which involved in three modules: complement and coagulation cascades, inflammatory response pathway and innate immune response (Figure 3C). Rev. Acer truncatum (purpleblow maple) is a woody tree species that produces seeds with high levels of valuable fatty acids (especially nervonic acid). For the sake of minimizing animal consumption and the importance of NA for human health, extraction of NA from plants has become a research hotspot. 6 111 doi: 10.1038/s41438-019-0194-7, Wang, X.Y., Fan, J.S., Wang, S.Y. doi: 10.1097/WNR.0000000000001242, Zhang, X., Meng, X., Chen, Y., Leng, S. X., and Zhang, H. (2017). Specifically, four mixed peptides were separated in the Xbridge BEH300 C18 column (Waters Milford, MA, United States) by HPLC (UltiMate 3000 UHPLC; Thermo Fisher Scientific, Waltham, MA, United States). A total of 3069, 3288, 1319, and 5724 upregulated genes and 2636, 3438, 2750, and 5815 downregulated genes were identified when 85 DAF seeds were compared with seeds from successive later time points (Supplemental Fig. There are many natural products which are found to delay aging and benefit cognition. Food Chem. (2016). 1 g of total RNA was used to synthesize cDNA. Order) cheap price pure vehetable oil Premium Safflower Seed Oil. & Shang, Q.M. The reaction was terminated by hydroxylamine. 2). D-Galactose-induced accelerated aging model: an overview. Docosahexaenoic acid dietary supplementation enhances the effects of exercise on synaptic plasticity and cognition. is the corresponding author. The link was not copied. & Guy, R.D. Place navigation impaired in rats with hippocampal lesions. The main ingredients of acer truncatum seed oil were listed in Table 1, which included eicosapentaenoic acid, DHA, nervonic acid, linoleic acid, -linolenic acid and vitamin E, which all reported to benefit for the cognition. Webtreated with saline (0.01 ml/g/day) or acer truncatum seed oil (0.01 ml/g/day) for 1 month, respectively. Additionally, the C16:0-ACP or C18:0-ACP can be further catalyzed by SAD to generate C16:1-ACP or C18:1-ACP; three SAD genes were identified and highly expressed, especially SAD1 (Figs.

Only 20 top suggestions will be shown. All data were expressed as mean SEM, n = 3 mice/group. In short, the expression trends of WRI1 and LEC1.1 increased and those of FUS3 and ABI3 decreased. 1. IL-1 forward primer 5- GCACTACAGGCTCCGAGATGAA-3 and reverse primer 5-GTCGTTGCTTGGTTCTCCTTGT-3. The oil component results showed that 85 DAF was the key time point for FA synthesis during seed development. Prog. The relative expression levels of genes were normalized with the internal reference gene Actin (Wang et al., 2018). In addition, we identified 20 DEGs associated with linoleic acid metabolism. Interestingly, in 115 vs. 85 DAF comparisons, 15 and 4 DEGs were significantly enriched in -linolenic acid and the linoleic acid metabolism pathway, respectively. 50 825 838 doi: 10.1111/j.1365-313X.2007.03092.x, Chen, D.J., Yan, L.H., Li, Q., Zhang, C.J., Si, C.L., Li, Z.Y., Song, Y.J., Zhou, H., Zhang, T.C. & Zhang, Y.L. IL-6 forward primer 5-AGTGGCTAAGGACCAAGAC-3 and reverse primer 5- ATAACGCACTAGGTTTGCCGA-3. The relative expression levels of genes were normalized with the internal reference gene Actin (Wang et al., 2018). All raw mass spectra were searched with the Uniprot-Mus musculus database (released in November, 2019) by Proteome Discoverer 2.1 software (Thermo Fisher Scientific, Waltham, MA, United States). Plant Growth Regul. In A. truncatum, six FAD genes were identified, which is less than that in A. thaliana (15), S. indicum (7), O. europaea (8), and G. max (15). Neurobiol.

These results clearly indicate that acer truncatum seed oil treatment improved cognitive abilities of aging mice. 2019 Comparative transcriptome analysis reveals an efficient mechanism for -linolenic acid synthesis in tree peony seeds Intl. It can adjust the immune system andincrease NK cell activity, improve the dual role of cellular immunity and humoral immunity and enhance Cycle. This work was funded by the Natural Science Foundation of China (32001357), the Independent Innovation Fund Project of Agricultural Science and Technology in Jiangsu Province (CX(20)3155), and the Technology Innovation and Extension Project of Forestry Science in Jiangsu Province (LYKJ[2018]14). In this study, nine GPAT genes were identified, but most of them were barely expressed; only two were relatively highly expressed during seed development. STAT1, CD9, and FINC were up-regulated in the aging control mice, but they were reversed by seed oil treatment, which found by proteomics analysis and proved by western blotting (Figures 3G,H). For the sake of minimizing animal consumption and the importance of NA for human health, extraction of NA from plants has become a research hotspot. WebComparing seed extract well prices. Plant 9 1667 1670 doi: 10.1016/j.molp.2016.09.014. (2016). & Li, Q.Z. Our free planting guide calculates the best dates for sowing seeds indoors and outdoors, and for transplanting seedlings into the gardenall customized to your location. doi: 10.1016/j.cmet.2016.05.011, Bitto, A., Ito, T. K., Pineda, V. V., LeTexier, N. J., Huang, H. Z., Sutlief, E., et al. & Xu, C. 2013 Dual role for phospholipid:diacylglycerol acyltransferase: Enhancing fatty acid synthesis and diverting fatty acids from membrane lipids to triacylglycerol in Arabidopsis leaves Plant Cell 25 3506 3518 doi: 10.1105/tpc.113.117358, Frandsen, G.I., Mundy, J. Aging brain: the effect of combined cognitive and physical training on cognition as compared to cognitive and physical training alone - a systematic review. The water surface was about 1.2 cm higher than the escape platform. The numbers of upregulated and downregulated genes were similar at different time points except for 145 vs. 85 DAF. truncatum 2016 iTAK: A program for genome-wide prediction and classification of plant transcription factors, transcriptional regulators, and protein kinases Mol. Commun. This is consistent with fact that the UFA proportions rapidly increased from 85 to 115 DAF. Nat.

The current study provides large-scale transcriptomics data for future research of lipid metabolism. & Seo, P.J. 26 1595 1611 doi: 10.1007/s10068-017-0218-8, Crowe, A.J., Abenes, M., Plant, A. Therefore, the gene expression of inflammation and anti-inflammation cytokines was detected by qPCR. (2011). doi: 10.7554/eLife.16351, Bonkowski, M. S., and Sinclair, D. A. DAF = days after flowering. doi: 10.1080/1028415X.2020.1831262, Li, Q., Chen, J., Yu, X., and Gao, J. M. (2019). The moderate aerobic exercise with vitamin E (VE) supplement increased antioxidant enzyme activities in the hippocampus of aged rats (Devi and Kiran, 2004), with reduced oxidative damage of proteins (Jolitha et al., 2006), and improved learning ability (Jolitha et al., 2009). 2017 Bioconversion of conjugated linoleic acid by Lactobacillus plantarum CGMCC8198 supplemented with Acer truncatum Bunge seeds oil Food Sci. (2009). Based Complement. doi: 10.1038/nrd4024, Lopez-Otin, C., Blasco, M. A., Partridge, L., Serrano, M., and Kroemer, G. (2013). Therefore, the results showed that 85 DAF was the key time point for FA synthesis. Cell. Fatty acid synthase (FAS) is a potential target in the treatment of both obesity and cancer. To further understand the basic molecular mechanisms of lipid biosynthesis and metabolism, RNA-seq data were generated from A. truncatum seeds at six developmental stages. The phenolic profile of SAT showed that the major phenolic acids were p-coumeric acid, gallic aid, cinamic acid, chlorogenic acid and ferulic acid, while catechin and rutin were the major flavonoids detected in the extract. Proteomic Profiles of the Early Mitochondrial Changes in APP/PS1 and ApoE4 Transgenic Mice Models of Alzheimers Disease. 3). As a food supplement, the acer truncatum seed oil may play a positive role in anti-aging. Sirtuin activators and inhibitors: promises, achievements, and challenges. Hort. 2019 Characterization of yuanbaofeng (Acer truncatum Bunge) samaras: Oil, fatty acid, and phytosterol content Ind. Chromosome-specific accumulation of aneuploidy in the aging mouse brain. The resulting FAs were analyzed using the gas chromatography-mass spectrometry (GC-MS) method as described by Jiao et al. doi: 10.1038/s41569-018-0064-2, Forsberg, L. A., Rasi, C., Razzaghian, H. R., Pakalapati, G., Waite, L., Thilbeault, K. S., et al. Forests 4; Industrial crops and products 4; Agricultural and forest meteorology 2; Lebensmittel-Wissenschaft + Technologie 2; The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway-related database was used to perform the DEG pathway enrichment analysis (Kanehisa et al., 2008). (2003). DHA diet in the Sprague-Dawley rats increased the expression level of BDNF, which acted on TrkB receptor signaling to attenuate learning disability in rats (Wu et al., 2008). In plants, the de novo synthesis of FAs occurs first in the plastid, with acetyl-CoA carboxylase (ACCase) catalyzing the biosynthesis of malonyl-CoA from acetyl-CoA.

These aging-related proteins were primarily involved in synapse, oxidative stress, inflammation, mitochondria, and apoptosis process, etc. Molecular inflammation: underpinnings of aging and age-related diseases.

Rep. 8 15504 doi: 10.1038/s41598-018-33999-3, Wang, X.J., Liang, H.Y., Guo, D.L., Duan, X.G., Jia, Q.S. A mini review of nervonic acid: source, production, and biological functions. On each trial, the mice started from one of the four quadrants facing the wall of the pool and ended when the animal climbed on the platform. A total of 28,438 genes were identified. Meanwhile, the oil treatment had no effects in the mRNA levels of anti-inflammatory cytokine IL-4 and IL-13 in aging mice (Figures 6E,F). It is native to China, Mongolia, Japan, and Korea. doi: 10.1007/s10522-019-09837-y, Bailey, C. H., Kandel, E. R., and Harris, K. M. (2015). & Yang, K.Q. Acer truncatum Bunge is now widely cultivated throughout the world. Before each experiment (2 h before), all mice were placed in the experiment room to allow them to adapt to the environment. GC (Gas Chromatography) analysis revealed that the oil contained approximately 6.22% nervonic acid. (2019). & Li, L.L. These results confirmed the reliability of the RNA-seq data obtained during our study. Proteins were transferred to the methanol infiltrated polyvinylidene fluoride (PVDF) membrane after separation. Ther. The seed oil of 138 accessions of 14 Acer truncatum (Aceraceae family) populations native to China were analyzed by pulsed nuclear magnetic resonance spectroscopy and gas chromatography-flame ionization detection. The oil content ranged from 17.81% to 36.56% (mean: 28.57%), which mainly consisted of 14 types of fatty acids.

These genes have the potential to increase the FA content and TAG synthesis in A. truncatum in the future. GO functional annotation was performed for the DEGs identified during the four comparisons with the 85 DAF sample (Supplemental Fig. 88 95 107 doi: 10.1111/tpj.13233, Zhao, W.H., Zhang, J.F., Wang, Z., Zhang, Y.X. 27, 639650. TFs have important roles in transcription and can modulate gene expression as regulators (Riechmann et al., 2000). A total of 4,309 proteins were identified in the entire hippocampus, and heatmap analysis of all the DE proteins was shown in Figure 3A, which completed by RStudio software. doi: 10.1016/S0197-4580(03)00112-X, Erickson, K. I., Miller, D. L., and Roecklein, K. A. *Correspondence: Xi Fei Yang, xifeiyang@gmail.com; Gong Ping Liu, liugp111@mail.hust.edu.cn, These authors have contributed equally to this work and share first authorship, https://doi.org/10.3389/fcell.2021.680386, http://proteomecentral.proteomexchange.org, https://www.frontiersin.org/articles/10.3389/fcell.2021.680386/full#supplementary-material, Creative Commons Attribution License (CC BY). Aging 13, 12671301. Thus, the oil may alleviate aging by decreasing the inflammation. doi: 10.1016/j.neuroscience.2008.05.061, Wu, R., Zhong, S., Ni, M., Zhu, X., Chen, Y., Chen, X., et al. & Maurice, M. 2000 The seed-specific transactivator, ABI3, induces oleosin gene expression Plant Sci. doi: 10.1093/hmg/dds375, Ferrucci, L., and Fabbri, E. (2018). The level of p-CREB/CREB was significantly up-regulated by acer truncatum seed oil in the aging mouse. Gerontol. Mol. WebTurf Center, Inc. 1409 Spencerville Road . Acer truncatum seed oil rich in nervonic acid was extracted using supercritical carbon dioxide. Alotaibi, S.S, Elseehy, M.M., Aljuaid, B.S. All mice aged 2 months or 20 months were randomly divided into control or acer truncatum seed oil treatment group (9 mice in each group). 12, 661684. By MCODE analysis, we found that these DE proteins by seed oil treated aging mice vs. aging control mice were involved in innate immune response, inflammatory response pathway and complement and coagulation cascades. The effects of enzyme type on the extraction yield of oil were studied, and the results showed that the oil yield obtained with pentosanase was higher than that obtained with the other enzymes. The 33 functional groups in the biological process, cellular component, and molecular function categories were classified according to the GO annotations of the DEGs. 2010 DEGseq: An R package for identifying differentially expressed genes from RNA-seq data Bioinformatics 26 136 138 doi: 10.1093/bioinformatics/btp612, Wang, L., Shen, W., Kazachkov, M., Chen, G., Chen, Q., Carlsson, A.S., Stymne, S., Weselake, R.J. & Zou, J.

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